2F-Stemcells

Stem Cells - The New Successful Therapy


LABORATORY

2F-Stemcells is a veterinary medical lab which is attached to the Tierärtzliche Klinik für Pferde Großwallstadt. With the help of somatic stem cells, we can treat equine tendon, ligament, and joint diseases.
LABORATORY
LABORATORY

Dr. Holger Fischer

We have a modern equipped lab for the preparation of stem cells. Also our highly qualified employees guarantee a continuous high quality product.

Prof. Dr. Dave Frisbie




OUR DIAGNOSTIC SERVICES


  • Stem cells - the new successful therapy
    In the last few years equine medicine has adopted a multitude of therapeutic methods. One of these is the use of stem cells for equine tendons, ligaments, and joints. As a general rule in veterinary medicine, it refers to the use of autologous multipotent cells from the bone marrow or adipose tissue. This does not refer to the ethically controversial growth and changes to embryonic stem cells.
  • Stem Cells from Bone Marrow
    • Bone marrow aspirates are taken from the ileum or sternum.
    • The aspirate is shipped overnight by a courier to 2F-Stemcells.
    • The bone marrow stem cells are prepared and propagated using laboratory techniques.
    • The number of cells is between 5 and 15 million depending on the degree of handling injury.
    • In general the preparation takes 2-3 weeks.
    • The cultivated bone marrow stem cells are in a frozen state and returned back to the treating veterinarian.

    Procedure to remove stem cells from the Ileum of a horse

    • Determine the puncture site (Fig. ½).
    • The red arrow marks the injection site for the puncture cannula.
    • With a small skin incision the puncture cannula can be brought to the right position (Fig. 3).
    • The heparinized 60 ml syringe is drawn up to the hub of the biopsy needle (Fig. 4).
    • Remove 8 – 10 ml bone marrow.
    • Repeat point number 4 with a second syringe (total volume bone marrow around 16 – 20 ml).
    • Remove the biopsy needle and close the skin wound.
    • Put the syringe cap on to avoid leakage (Fig. 5).
    • Remove 12 ml venous blood from the same horse (the serum will be cryopreserved until needed).
    • Send both the bone marrow aspirate and serum along with an identification note to 2F-Stemcells (see the shipment protocol).

    Click here for the detailed protocol describing retrieval of bone marrow.
    2F_Protokoll_Illeum.pdf

    Procedure to remove stem cells from the Sternum of a horse

    • Palpate the sternum between the beginning of the front leg and about 16 cm caudal.
    • This area of the sternum is now aseptically prepared (Fig. 6).
    • Through a small skin incision, (about 0.5 – 1 cm) penetrating the subcutaneous tissue, the puncture cannula is lead ventral into the sternum.
    • Remove the stylet and attach the heparinized syringe.
    • Remove 5 – 7 ml of bone marrow aspirate (Fig. 7).
    • Repeat the procedure with the second syringe. Move the cannula in the area 2-3 cm cranial or caudal.
    • Put the syringe cap on to avoid leakage.
    • Remove 12 ml venous blood from the same horse (the serum will be cryopreserved until needed).
    • Send both the bone marrow aspirate and serum along with an identification note to 2F-Stemcells (see the shipment protocol).

    Click here for the detailed protocol describing retrieval of bone marrow.
    2F_Protokoll_Sternum.pdf
  • General and Background Information
    Stem cells are cells from the body which are not yet differentiated. There are in a form which is not yet available for specialized use in organs (i.e. muscle cells, bone cells, etc.). Their later use is still open. The fate of the cell is decided by the surrounding environment in which it is found.

    Along with the origin, one can also differentiate two categories of stem cells:

    Embryonic Stem Cells

    Embryonic stem cells are propagated in vitro (outside of the body) from mammalian blastocysts. They build the precursors in the embryo for all cells. In principle, they can be propagated as undifferentiated cells.

    Adults (somatic) Stem Cells

    Adult (somatic) stem cells are available in the body after birth. Throughout the organisms life new specialized cells will continuously be made from these stem cells. Compared to embryonic stem cells they have limited self-renewing capabilities and also limited potential to differentiate. Somatic stem cells are found in bone marrow, liver, brain, and surprisingly high concentrations in adipose tissue. These cells are found in all living creatures and facilitate the use of autologous (naturally produced in the body) cells for different disease processes (i.e. treatment of leukemia in human medicine).


    New Approaches to treatment of tendons and ligaments of horses

    Noch vor einigen Jahren waren zur Therapie von Sehnenschäden nur die Injektion von Hyaluronsäure in das erkrankte Fasergewebe oder die Implantation spezieller Faserbänder bekannt. Diese Therapien haben sich jedoch nicht dauerhaft durchgesetzt bzw. zeigten häufig nicht den erwünschten Erfolg.

    A few years ago the only known treatment for tendon damage was injection of hyaluronic acid into fibrous tissue or the implantation of special fibrous bands. However these therapies have not been carried through the long term and have frequently not shown the desired results. The transfer of autologous bone marrow into chronic tendonitis and insertion desmopathies has been used in equine medicine for about three years now. This therapy produces a multitude of good results and gives a reason for hope that the concentration of stem cells could give better results. Approximately 0.001% - 0.01% of the nucleated cells in the prepared bone marrow are multipotent cells (stem cells).

    From now on we are substantially capable of improving this therapeutic application.
  • Laboratory
    Processing fat stem cells

    Processing fat stem cells

    2F-Stemcells is a veterinary medical lab which is attached to the Tierärtzliche Klinik für Pferde Großwallstadt. With the help of somatic stem cells, we can treat equine tendon, ligament, and joint diseases.
    Equine Veterinary Hospital

    Equine Veterinary Hospital

    We have a modern equipped lab for the preparation of stem cells. Also our highly qualified employees guarantee a continuous high quality product.
  • Indications
    Stem cell therapy is indicated by other forms of tendonitis:
    • The superficial flexor tendon
    • The deep flexor tendon
    • The suspensory apparatus branch and origin
    • The biceps tendon
    • The deep tendon of the navicular bone
    Through both the improved ultrasound techniques as well as magnetic resonance imaging, more differential diagnoses are made in the hoof area. Which was typically an area difficult to show. This applies for all insertion desmopathies (elevation or irritation of ligaments at their attachment to bone). Great results have been seen from implanting stem cells prepared from bone marrow into arthritic joints.
    Additional common locations of insertion desmopathies:
    • The suspensory apparatus proximal and distal
    • The collateral ligaments of the coffin bone
    • The unpaired distal sesamoid bones-coffin bone
    • The nuchal ligament
    • The collateral ligaments of the coffin joint
    The described methods in comparison have shown to be as of now an outstanding prognosis for cure even in older diseases. Both the scientific security and also the continuing improvements are guaranteed through our highly qualified research team, Dr. Dave Frisbie and Dr. John Kisiday. This is through relevant publications and research from the body of experts.



VISIT US

9 Niedernberger Strasse
Grosswallstadt 63868

OPENING HOURS

24-hour service
365 days a year

CONTACT

Phone
(0) 6022 / 265 970
Fax
(0) 6022 / 258 66

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